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five–4 μmol/L variety,[156][157] the concentration of serotonin in synaptic vesicles was calculated at 270 mM.[182] next vesicular release, the resulting focus of serotonin within the synaptic cleft, to which serotonin receptors are uncovered, is believed to become about three hundred μmol/L. Hence, whilst in vitro receptor binding affinities
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